The College of Education for Pure Sciences, Diyala University discussed a master dissertation on the effect of certain physical treatments on the coumarin content of rue callus by the postgraduate student, Ms. Israa Jassim Mohammed.

The dissertation addressed the importance of medicinal plants that possess therapeutic properties capable of treating several diseases, due to the active compounds they contain, which are concentrated in the plant's parts.

The dissertation demonstrated that callus exposed to a short thermal shock (30°C for 5 minutes) was the most effective in increasing the wet and dry weight of the callus compared to the control sample.

The dissertation also demonstrated a clear effect of thermal treatments on the total chlorophyll concentration in plant tissues.

The dissertation concluded that physical shocks can be used as an effective means of stimulating callus growth and increasing the production of active compounds in rue, representing an important step in supporting biotechnological applications to produce plant extracts with pharmaceutical value.