College of Science, Mustansiriyah University discussed a Ph.D. thesis on the anti-biofilm and anti-cancer activity of arginase purified from Pseudomonas aeruginosa bacteria isolated from soil and wastewater by the postgraduate student, Mrs. Zainab Abbas Abdel.

The thesis highlighted isolating and diagnosing Pseudomonas aeruginosa bacteria from various sources, purifying the arginase enzyme, extracting it and characterize it, identifying the gene responsible for its production from bacteria and studying its effect on both cancer and the formation of biofilms mediated by some types of bacteria, as well as identifying the effect of heavy metals on the effectiveness of arginase.

The thesis aimed at extracting the arginase enzyme and purifying it from the Pseudomonas aeruginosa bacteria by knowing the optimal conditions that include temperature, pH, and genetic diagnosis based on PCR technology to determine the gene responsible for producing the enzyme and studying the effect of heavy metals on the properties of the purified enzyme, not to mention investigating its effect on breast cancer and in biofilms formed by pathogenic bacteria isolated from various sources.

The thesis demonstrated that the purified arginase enzyme affects the effectiveness of biological membranes and leads to their conversion from strong membranes to weak or non-productive membranes, the enzyme also has a clear effect on cancer cells of the type (AMJ-13) compared to normal cells (REF), the percentage of killing cancer cells was (89%), while the percentage of killing normal cells was less than (40%).