College of Science, Mustansiriyah University discussed a master dissertation on a molecular and immunological study of Salmonella entericaserovar Typhi bacteria isolated from typhoid fever patients by the postgraduate student, Ms. Mona Sabah Daoud.

The dissertation aimed at determining the presence of some virulence genes (viaB, staA, cdtB, orfL) in Salmonella Typhi isolates using the polymerase chain reaction (PCR) technique and analyze their relation to the levels of interleukin 4 and 18 in patients with typhoid fever.

The dissertation highlighted the isolation and diagnosis of the studied bacteria, included the use of microbiological methods to conduct the polymerase chain reaction by using the 16S rRNA gene, testing the sensitivity to antibiotics, detecting the four virulence genes by PCR, and evaluating the role of interleukin 4 and 18 in typhoid fever patients and its relation with virulence genes.

The dissertation demonstrated a significant increase in the levels of interleukin 4 and 18 in patients compared to the control group, indicating the association of these immune factors with virulence genes in bacteria and their effect on patients with typhoid fever.

The dissertation reviewed the resistance of bacteria to some antibiotics, which reflects the importance of monitoring the effectiveness of treatments used to combat the disease.