College of Agriculture, University of Diyala discussed a master dissertation on diagnosing of Rhizobium bacteria used for the biological and root treatment of soil contaminated with oil derivatives using the PCR technique by the postgraduate student, Mrs. Zainab Adnan Hussein.

The dissertation reviewed four experiments, as well as isolating and diagnosing of Rhizobium bacteria.

The dissertation concluded that local bacterial isolates are efficient in analyzing gas oil, and that increasing levels of gas oil led to a significant decrease in plant height, plant dry weight, root length, number and weight of root nodes and obtaining a local isolate for the first time in Iraq by means of PCR, which proved its efficiency with the Rhizobium isolate in most of the studied traits.

The dissertation recommended the use of other bacterial isolates with fungal isolates to increase the amount of microbial decomposition and preserve the isolates, especially the new isolate and test and add them to the plant.